Journal: The International journal of developmental biology

Article Title: A screen of kinase inhibitors reveals a potential role of Chk1 in regulating Hydra head regeneration and maintenance.

doi: 10.1387/ijdb.210087yl

Figure Lengend Snippet: Fig. 1. Schematic illustration of the 80 kinase inhibitor screen. Three kinase inhibitors (KIs) were tested per 12-well plate. For each plate, 60 Hydra gastric regions were isolated by removing the head and foot. For each treatment, 15 gastric regions were rinsed with the respective solution (KI #1, KI #2, KI #3, or control) before dividing them into 3 wells (5 gastric regions per well) containing the respective exposure solutions. Control replicates were exposed to 1% DMSO alone and included on every plate.

Article Snippet: The use of kinase inhibitors to identify potential novel regulators of Hydra regeneration To identify novel signaling pathways underlying Hydra regeneration, an unbiased screen of 80 kinase inhibitors (Tocriscreen Kinase Inhibitor Toolbox Cat. No. 3514) was performed.

Techniques: Isolation, Control

Journal: The International journal of developmental biology

Article Title: A screen of kinase inhibitors reveals a potential role of Chk1 in regulating Hydra head regeneration and maintenance.

doi: 10.1387/ijdb.210087yl

Figure Lengend Snippet: Fig. 2. Morphological categorization following exposure to the 80 kinase inhibitors. In total, 4 major morphologies were observed in Hydra following exposure to the 80 kinase inhibitors: (A) complete regeneration, (B) partial regeneration – the presence of shortened tentacles and/or body column, (C) arrested regeneration - no formation of a head and foot, and (D) disintegration - the breakdown of tissues into debris. Scale bar in (A) represents 1000 mm and applies to all panels.

Article Snippet: The use of kinase inhibitors to identify potential novel regulators of Hydra regeneration To identify novel signaling pathways underlying Hydra regeneration, an unbiased screen of 80 kinase inhibitors (Tocriscreen Kinase Inhibitor Toolbox Cat. No. 3514) was performed.

Techniques:

Journal: The International journal of developmental biology

Article Title: A screen of kinase inhibitors reveals a potential role of Chk1 in regulating Hydra head regeneration and maintenance.

doi: 10.1387/ijdb.210087yl

Figure Lengend Snippet: Fig. 3. Drug Exposure Impact of the 80 kinase inhibitors on Hydra regeneration. Kinase inhibitors were grouped by the main signal transduction pathways they are presumed to target. Drug Exposure Impact (DEI) values on regeneration were calculated as the difference in regeneration scores obtained for control and Hydra exposed to 5 mM kinase inhibitor. DEI values for complete regeneration ranged between -1.07 and 1.2; partial regen eration ranged between -4.4 and 0.6; arrested regeneration ranged between -7.67 and -4; and disintegration ranged between -10 and -7.8. Error bars represent ± 2x standard error.

Article Snippet: The use of kinase inhibitors to identify potential novel regulators of Hydra regeneration To identify novel signaling pathways underlying Hydra regeneration, an unbiased screen of 80 kinase inhibitors (Tocriscreen Kinase Inhibitor Toolbox Cat. No. 3514) was performed.

Techniques: Transduction, Control

Journal: Nature Communications

Article Title: Poziotinib for EGFR exon 20-insertion NSCLC: Clinical efficacy of the phase 2 ZENITH trial and differential impact of EGFR exon 20 insertion location on sensitivity

doi: 10.1038/s41467-025-61817-8

Figure Lengend Snippet: a – f Dot plots of average IC 50 values for Ba/F3 cell lines expressing various EGFR exon 20 insertion mutations ( N = 22 cell lines) treated with the indicated inhibitors compared to the amino acid residue of the EGFR exon 20 insertion mutation. The red dashed line indicates the average IC 50 value for the TKI against Ba/F3 cells expressing WT EGFR + 10 ng/ml EGF. Dots are representative of the average IC 50 value determined in biological triplicate. a – f Data was fit to a linear regression model, and a two-tailed Pearson correlation was determined. Each specific mutation was denoted as a colored dot, and a key was provided to the right of the figure. a mobocertinib, ( b ) afatinib, ( c ) zipalertinib (CLN-081), ( d ) osimertinib, ( e ) Tarlox-TKI, ( f ) poziotinib. g Boxplots were shown and t test was used to compare the IC50 of each drug in near- ( n = 17) vs far-loop ( n = 5) mutations.

Article Snippet: Tyrosine kinase inhibitors were purchased from Selleck Chemicals, with the exception of Tarlox-TKI which was purchased from MedChem Express.

Techniques: Expressing, Residue, Mutagenesis, Two Tailed Test